Figure 2. Tiam1 deletion from spinal dorsal horn neurons prevents neuropathic pain development.

(A) Intra-spinal dorsal horn microinjection of rAAV8-hSyn-GFP (GFP).

(B) Representative blots and quantification show that injection of rAAV8-hSyn-Cre-GFP (Cre) downregulates Tiam1 protein levels in the spinal dorsal horn of Tiam1^fl/fl mice. GAPDH was used as the loading control. Mann-Whitney U-test (n = 4).

(C and D) Tiam1 deletion from spinal dorsal horn neurons prevents SNI-induced neuropathic pain. Time course for changes in von Frey withdrawal thresholds and time flicking with acetone evaporation of Tiam1^fl/fl mice infected with rAAV8-hSyn-GFP (GFP) or rAAV8-hSyn-Cre-GFP (Cre) before and after SNI. Two-way ANOVA analysis followed by Dunnett’s post-hoc test (n = 7–8 mice/group. von Frey: P < 0.0001, F_6,84 = 7.477; acetone: P < 0.0001, F_6,91 = 8.992).

(E and F) Tiam1 ablation from spinal dorsal horn neurons precludes paclitaxel (Taxol)-induced neuropathic pain. Two-way ANOVA analysis followed by Dunnett’s post-hoc test (n = 8 mice/group. von Frey: P < 0.0001, F_5,84 = 6.408; acetone: P < 0.0001, F_5,84 = 8.940).

(G and H) Tiam1 deletion from spinal dorsal horn neurons inhibits streptozotocin (STZ)-induced neuropathic pain. Two-way ANOVA analysis followed by Dunnett’s post-hoc test (n = 9–10 mice/group. von Frey: P < 0.0001, F_4,84 = 14.69; acetone: P < 0.0001, F_4,70 = 14.90).

Data are presented as means ± s.e.m. **P < 0.01, ***P < 0.001, ****P < 0.0001.

See also Figures S3, S4, and S5.