KEY RESOURCES TABLE

REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Rabbit anti-Tiam1	Santa Cruz	SC-872
Sheep anti-Tiam1	R&D Systems	AF5038
Rabbit anti-Rac1	Cell Signaling	4651
Mouse anti-Actin	Millipore	MAB1501
Rabbit anti-GluN1	Sigma	G8913
Rabbit anti-GluN1	Abcam	AB52177
Rabbit anti-GluN2A	Thermo Fisher	PA5-35377
Mouse anti-GluN2B	NeuroMab	75-002
Mouse anti-GluA1	NeuroMab	75-327
Rabbit anti-GluA2	Millipore	Ab10529
Rabbit anti-PSD-95	Abcam	Ab18258
Rabbit anti-EphB	Thermo Fisher	PA5-39736
Rabbit anti-TrkB	Abcam	Ab187041
Rabbit anti-Caspase 3	Cell Signaling	9662
Mouse anti-GAPDH	Santa Cruz	Sc-47724
Peroxidase Donkey anti-rabbit IgG	Jackson Immuno Res	711-035-152
Peroxidase Goat anti-mouse IgG	Jackson Immuno Res	115-035-003
Chemicals, peptides, and recombinant proteins
NSC23766	Tocris	2161
NMDA	Tocris	0114
AMPA	Tocris	0254
Streptozotocin	Sigma-Aldrich	S0130
L-Glutamate	Sigma-Aldrich	49621
Bicuculline	Sigma-Aldrich	14343
(2R)-amino-5-phosphonopentanoate (AP5)	Abcam	ab120003
Spermine	Sigma-Aldrich	85590
Protease and phosphatase inhibitors	Thermo Fisher	78440
4–15% Mini-PROTEAN Precast Protein Gels	Bio-Rad	4560000
Pierce Fast Western Blot Kit, ECL Substrate	Thermo Fisher	35050
RIPA Lysis Buffer	Thermo Fisher	89900
Experimental models: Organisms/strains
Tiam1 floxed mice	Cheng et al., 2021	PMID: 33328293
CaMKIIα-Cre mice	Jackson Laboratory	005359
Advillin-Cre mice	Jackson Laboratory	026516
vGluT2-Cre mice	Jackson Laboratory	016963
vGat-Cre mice	Jackson Laboratory	017535
Recombinant DNA
rAAV8-hSynapsin-eGFP	UNC Vector core	N/A
rAAV8-hSynapsin-Cre-eGFP	UNC Vector core	N/A
rAAV8-hSynapsin-Rac1Q61L-GFP	BCM Vector core	N/A
rAAV8-pCAG-DIO-eGFP	UNC Vector core	N/A
Software and algorithms
Prism 9.0	GraphPad Software	https://www.graphpad.com/
ImageJ	ImageJ	https://imagej.nih.gov/ij/download.html
Clampfit 10.0 software	Axon Instruments	www.moleculardevices.com