Figure 4. Skeletal Muscle-Specific deletion of the AHR Preserves Ischemic Muscle Mitochondrial Energetics in Mice with CKD.

(A) Relationship between JO₂ and ΔG_ATP when mitochondria were fueled with pyruvate and malate and quantification of the conductance (slope of JO₂ and ΔG_ATP relationship) and uncoupled respiration rates in male mice (n=8 AHR^fl/fl male CON and 12 CKD, 11 AHR^mKO male CON and 14 CKD). (B) Relationship between JO₂ and ΔG_ATP when mitochondria were fueled with octanoylcarnitine and malate and quantification of the conductance and uncoupled respiration rates in male mice (n=11 AHR^fl/fl male CON and 13 CKD, 10 AHR^mKO male CON and 13 CKD). (C) Relationship between JO₂ and ΔG_ATP when mitochondria were fueled with pyruvate and malate and quantification of the conductance and uncoupled respiration rates in female mice (n=11 AHR^fl/fl female CON and 13 CKD, 10 AHR^mKO female CON and 9 CKD). (D) Relationship between JO₂ and ΔG_ATP when mitochondria were fueled with octanoylcarnitine and malate and quantification of the conductance and uncoupled respiration rates in female mice (n=11 AHR^fl/fl female CON and 9 CKD, 9 AHR^mKO female CON and 7 CKD). (E) Western blotting membrane of mitochondrial electron transport system proteins (n=8/group/sex/genotype). (F) Quantification of mitochondrial protein abundance in male mice n=8/group/sex/genotype). (G) Quantification of mitochondrial protein abundance in female mice n=8/group/sex/genotype). (H) qPCR analysis of mitochondrial transcripts in male mice (n=6/group). (I) qPCR analysis of mitochondrial transcripts in female mice (n=6/group/sex/genotype). Statistical analyses performed using two-way ANOVA with Sidak’s post hoc testing for multiple comparisons. Error bars represent the standard deviation.