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. Author manuscript; available in PMC: 2024 Jul 7.
Published in final edited form as: Circ Res. 2023 Jun 16;133(2):158–176. doi: 10.1161/CIRCRESAHA.123.322875

Figure 6. AHRmKO mice with CKD have preserved paracrine vasculogenic signaling between myofibers and vascular cells.

Figure 6.

(A) Circle plots showing the overall intercellular communication occurring in AHRfl/fl and AHRmKO muscles. (B) Circle plots showing VEGF and NOTCH signaling communication in AHRfl/fl and AHRmKO muscles. (C) Ranked significant ligand-receptor communications for relative information flow between AHRfl/fl and AHRmKO muscles. (D) Enriched outgoing and incoming signaling patterns according to cell type based on signaling strength. (E) Targeted angiogenic and vascular reactivity qPCR analysis on total RNA from the ischemic hindlimb muscles (n=6/group/sex/genotype). (F) Analysis of the protein abundance of secreted angiogenic growth factors in conditioned media from C2C12 myotubes (n=4/group). Analyses in A-D were performed using CellChat. Panel E analyses involved a two-way ANOVA with Sidak’s post hoc testing for multiple comparisons. Panel F was analyzed with a Mann-Whitney test. Error bars represent the standard deviation.