Figure 4.

Dual pS193/pT196 tau and total tau expression in brain tissue of four month post-SE rats. (A) Representative western blot showing greatly decreased dual pS193/pT196 tau expression in CA3 hippocampus in chronic epilepsy using the AT8 Ab. (B) Representative western blot demonstrating a modest reduction in total tau expression in CA3 hippocampal regions using the Tau-5 Ab in chronic epilepsy. (C) Group data showing changes in tau expression in chronically epileptic rats at four months post-SE in the whole hippocampal formation, CA1 hippocampus, CA3 hippocampus, and somatosensory cortex (SSC) compared to age-matched naïve rats. Values shown are individual data points; boxes denoting median, 75^th and 25^th percentiles; whiskers showing 95^th and 5^th percentiles; and mean value (diamonds). Significant decreases in dual pS193/pT196 tau were observed in all hippocampal regions in epilepsy (green box plots). The only change in total tau expression was the decrease observed in the CA3 hippocampus (red box plots). Reductions in fractional pS193/pT196 tau expression (pS193/pT196 tau/total tau) were detected in the whole hippocampal formation and in the CA3 hippocampus (blue box plots). No change in tau expression was seen in the SSC.