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. Author manuscript; available in PMC: 2024 Jul 1.
Published in final edited form as: Neuroscience. 2023 May 2;522:42–56. doi: 10.1016/j.neuroscience.2023.04.020

Figure 6.

Figure 6.

Localization of pS193/pT196 tau protein in the hippocampal CA1 region within age-matched naïve (A-D) and chronic 4 mo. post-SE (E-H) rats by AT8 Ab staining. pS193/pT196 tau staining is shown in green, and nuclear staining by 4′,6-diamidino-2-phenylindole (DAPI) is shown in blue. Scale bars = 20 μm. (A-D) The lower magnification image (A) represents a larger scale view of pS193/pT196 tau staining within the CA1 hippocampus of naïve rats. The boundary between CA1 and CA3 hippocampal subregions is demarcated. Note the qualitative increased dendritic staining within the CA3 hippocampus (*) compared to that within the CA1 hippocampus at the transition. pS193/pT196 tau staining examples from three different naïve rats of a magnified view within the CA1 subregion are displayed in (B-D). The most abundant expression of pS193/pT196 tau was observed in the CA1 somata within the stratum pyramidale (p) layer; some staining was present in the dendrites in the stratum radiatum layer (r), while little to no staining was observed in the axons within the stratum oriens (o). (E-F) The lower magnification image (E) represents a larger scale view of pS193/pT196 tau staining within the CA1 hippocampus of 4 mo. post-SE of chronically epileptic rats with the boundary between the CA1 and CA3 hippocampal subregions labelled. pS193/pT196 tau staining examples from three different chronically epileptic rats of a magnified view within the CA1 subregion are displayed in (F-H). The distribution of pS193/pT196 tau within the CA1 hippocampus in chronically epileptic rats was similar to that in age-matched naïve rats: the greatest expression was observed within the stratum pyramidale (p) layer; lesser extent in the stratum radiatum layer (r), while little to no staining within the stratum oriens (o). Overall, pS193/pT196 tau staining in the CA1 hippocampal subregion of chronically epileptic rats qualitatively was less than that of naïve rats.