Prenatal PAH exposure lowered Pparγ methylation among grandoffspring (F2) mice at PND28, but not among offspring (F1) mice at PND28. Percent CpG methylation in mammary tissue of three sites (CpG-189, CpG-205, and CpG-241) in the Pparγ promoter region are shown following control (open circles) and PAH exposure (open squares) among F1 PND28 (control n = 7; PAH n = 10) and F2 mice at PND28 (control n = 10; PAH n = 10). Mean and 95% confidence intervals are marked. The p values for the association between prenatal PAH exposure and Pparγ methylation are shown for significant and borderline significant associations. Analyses were performed by t test. Abbreviations: PND: postnatal day, F1: first generation offspring, F2: second generation offspring.