Three independent experiments (E1, E2, and E3) were performed with two batches of HA (B1 and B2) using U87 and LN229 mono-spheres encapsulated in Col-HA gels: A) experiment design and B) model configuration. (C, D) Sphere diameter, migration capacity, and adjusted migration distance (ad-distance) of C) U87 and D) LN229 mono-spheres are presented as average ± standard error. N = 4–8. (E, F) Principal component analysis (PCA) plots of E) U87 and F) LN229 mono-spheres shows separation of individual spheres in three experiment groups at the last day of culture (day 7 for U87 and day 9 for LN229).