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. 2023 Jun 26;14:1212149. doi: 10.3389/fmicb.2023.1212149

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Copyright © 2023 Lei, Hanks, Bao and Liu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Anti-M protein western blots show GAS variants with loss of M protein production. (A) Anti-M protein blot of MGAS2221, MGAS5005 and SF370 and their gene deletion mutants. Lanes: 1, MGAS2221; 2, GAS1213 (MGAS2221ΔspyCEP); 3, GAS1473 (MGAS2221ΔsclA); 4, GAS1498 (MGAS2221Δspy1118); 5, MGAS5005; 6, GAS1133 (MGAS5005Δsse); 7, SF370; 8, GAS827 (SF370ΔcovS); and 9, GAS1458 (SF370ΔsclA). (B) Anti-M protein blot of MGAS2221 (lane 1) and its derivative isolates from in vitro culture after 15 passages (lanes 2 to 14).