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Slipped-strand mispairing within the c.1571C[8] polycytidine tract of mga turns off M protein production. (A) DNA sequencing chromatogram shows 8\u00B0C repeats of the polycytidine tract near the 3′ end of mga in MGAS2221 but 7\u00B0C repeats of the tract in its M- variant (GAS1285) that led to a Mga-M1 protein fusion protein. (B) In trans complementation of the M- variant with pDCBB-mga but not pDCBB (vector control) restored M protein production.
