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. 2023 Jun 26;14:1212149. doi: 10.3389/fmicb.2023.1212149

Figure 3.

Figure 3

Selection of M+ GAS isolates from M− GAS variants in infection sites of mice. Mice were inoculated subcutaneously with M− c.1574C[7] variants of MGAS2221 or MGAS2221 ΔsagA, and GAS isolates from the infection site of the skin were tested for M protein production by Western blotting analyses. (A) Anti-M protein western blot of GAS isolates. Lanes: 1, MGAS2221; 2–14, isolates from skin infection site of M− MGAS2221 ΔsagA. (B) The C-terminus Amino sequences starting from residue 501 for wild-type M1 Mga (c.1574C[8]) and its c.1574C[7] and c.1574C[6] variants. The sequences in red are different from that of the wild-type Mga, and [62AA] and [M protein] for c.1574C[7] variant represent amino acid sequence of the 66 amino acid residues encoded by the sequence between mga and emm and the sequence of the M protein, respectively. The numbers 529, 1,075 and 542 are the total number of amino acid residues in wild-type Mga and its c.1574C[7] and c.1574C[6] variants, respectively. (C) Relative emm mRNA levels of MGAS2221 (control), M− GAS1806, and M+ M497 and M406 and M− M405 isolated from GAS1806 infection site in the skin of mice. The polymorphism of the mga polycytidine tract is indicated.