Table 1.
Data collection, phasing and refinement statistics, related to Figure 6
| Native SpAtg44 | Selenomethionine-labeled SpAtg44V42M | |
|---|---|---|
|
| ||
| Data collection statistics | ||
| Beamline | SPring-8 BL32XU | SPring-8 BL32XU |
| Wavelength (Å) | 1.0000 | 0.9790 |
| Space group | P4122 | F432 |
| Cell dimensions | ||
| a, b, c (Å) | 82.364, 82.364, 113.427 | 166.322, 166.322, 166.322 |
| a, b, c (°) | 90.00, 90.00, 90.00 | 90.00, 90.00, 90.00 |
| Resolution range (Å) | 46.71–1.58(1.64–1.58) | 50.16–2.18(2.25–2.18) |
| R merge (I) | 0.260(1.124) | 0.266(1.749) |
| I / σ(I) | 5.2(0.5) | 8.7(0.7) |
| Completeness (%) | 97.3 (78.8) | 100.0(100.0) |
| Phasing statistics | ||
| Resolution range (Å) | 41.58–2.18 | |
| No. of sites | 3 | |
| Mean figure of merit | 0.27 | |
| Refinement statistics | ||
| Resolution range (Å) | 46.71–1.58 | |
| No. of reflections | 52606 | |
| Rwork / Rfree | 0.196/0.226 | |
| r.m.s.d.a from ideality | ||
| Bond lengths (Å) | 0.006 | |
| Bond angles (°) | 0.823 | |
a
root-mean-square deviation