Table 2.
Bacteriological and biochemical characteristics of Klebsiella aerogenes strain-RD99.
| Tests performed | Test result (Strain RD99) |
|---|---|
| Colony morphology | Medium size, glossy, mucoid colony |
| Gram stain | Gram negative bacilli |
| Catalase test | Positive |
| Oxidase test | Negative |
| Kligler's Iron Agar (KIA) test | |
| a. Acid production in slant | Positive |
| b. acid production in butt | Positive |
| c. Hydrogen sulfide production (H2S) | Negative |
| d. Gas production | Positive |
| Motility Indole Ureas Test (MIU) | |
| a. Motility | Positive (After 36 h) |
| b. Indole Production | Negative |
| c. Urea hydrolysis | Negative |
| Simmons citrate reaction test | Positive |
| Acetate | Negative |
| Sugar fermentation | |
| a. Glucose | Positive |
| b. Lactose | Positive |
| c. Sucrose | Positive |
| d. Maltose | Positive |
| e. Mannose | Positive |
| f. Arabinose | Positive |
| g. Sorbitol | Positive |
| h. Mannitol | Positive |
| i. Inositol | Positive |
| j. Esculin | Positive |
| Nitrate reduction | Positive |
| Gelatine liquefaction | Negative |
| ONPG | Positive |
| Vogas-proskauer | Positive |
| Lysine decarboxylase | Positive |
| Ornithine decarboxylase | Positive (After 36 hours) |
| Arginine Dihydrolase | Negative |
| Haemolysis on Blood Agar | Gamma-hemolysis |
| Growth on agar media | |
| a. MacConkey Agar | Light pink color colony |
| b. SS agar agar | Light pink color colony |
| c. CHROMagar™ Orientation | Metallic blue color colony |
| d. Blood agar | Gray white colony |
| e. Gelatin agar | White colony |
| Growth temperature | 26–40°C |
| API 20E number | 5305773 (Detect Enterobacter aerogenes with 96% pro bability) |