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. Author manuscript; available in PMC: 2024 Jun 15.
Published in final edited form as: Mol Cell. 2023 May 30;83(12):2003–2019.e6. doi: 10.1016/j.molcel.2023.05.010

Figure. 3.

Figure. 3

RBM33 plays an essential role in ALKBH5 loading to the substrate. (A and B) Amino acids in the interaction interface between RBM33 and ALKBH5 based on the HDOCK prediction. (C and D) Co-IP analysis as indicated. (E) m6A-modified RNA oligos pull-down analysis as indicated. (F and G) Dot-blot and RNA m6A methylation quantification analysis of global mRNA m6A methylation as indicated, G shown as mean ± SEM. (H) m6A-modified RNA oligos pull-down analysis as indicated. (I and J) Dot-blot and RNA m6A methylation quantification analysis of mRNA m6A demethylase activity of ALKBH5 K132A, E153A, and T265A mutants, J shown as mean ± SEM.