(A) Airyscan images of WT, GS28 KO and COG4 KO cells stained for GM130, SNAP29, and STX5 show redistribution of SNAP29 to the Golgi region in GS28 KO and COG4 KO cells. (B) There is a significant increase in the colocalization between SNAP29 and STX5 in GS28 KO and COG4 KO cells compared to WT cells. (C) WB analysis of STX5 IP in WT, GS28 KO, COG4 KO and COG7 KO cells shows increased coIP of alternate partners SNPA29 and VTI1B in COG depleted cells. (D) There is more than two-fold increase of SNAP29 coIPed with STX5 in COG4 depleted cells. *p<0.05, **p<0.001, ***p<0.0001