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. Author manuscript; available in PMC: 2024 Aug 1.
Published in final edited form as: Traffic. 2023 Jun 21;24(8):355–379. doi: 10.1111/tra.12903

Figure 8: Displacement of STX5 from the Golgi impairs Golgi glycosylation more significantly in GS28 KO compared to WT.

Figure 8:

(A-D) Flow-cytometry analysis of Retro2 treated WT and GS28 KO cells surface stained with GNL-647 and HPA-488. The histograms are representative of biological triplicates and at least 30,000 cells per replicate. GNL and HPA staining intensities are higher in WT and GS28 KO cells treated with Retro2 compared to their untreated counterparts indicating N-glycosylation and O-glycosylation defects. (E) Staining the total cell lysate with fluorescently labeled GNL and HPA indicates accumulation of underpocessed N- but not O- glycans in cells treated with Retro2. (F, G) Treatment with Retro2 affects the abundance of Golgi glycosylation enzymes ***p<0.0001