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. Author manuscript; available in PMC: 2024 Aug 1.
Published in final edited form as: Traffic. 2023 Jun 21;24(8):355–379. doi: 10.1111/tra.12903

Figure 9: Triple knockout (TKO) of GS28, SNAP29 and VTI1B results in severe glycosylation defects.

Figure 9:

(A) Flow cytometry analysis of WT, GS28 KO and TKO cells using fluorescent lectins. Histograms showing the fluorescence intensity of intact wild type and mutant cells labeled with the indicated lectins for 30 mins on ice (B) Histograms showing the fluorescence intensity of WT, TKO and TKO rescued cells surface labeled with the GNL for 30mins on ice. (C) Bar graphs showing the mean fluorescence intensity for GNL indicate a decrease in GNL binding in the TKO cells expressing either GS28-YFP or GFP-SNAP29 or GFP-VTI1B suggesting that each of these SNAREs can partially rescue N-glycosylation defects in the TKO cells. Monosaccharide symbols follow the Symbol Nomenclature for Glycans (SNFG). (107)