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. Author manuscript; available in PMC: 2024 Jul 13.
Published in final edited form as: Neurosci Lett. 2023 May 18;809:137305. doi: 10.1016/j.neulet.2023.137305

Figure 1:

Figure 1:

Developmental increases in AMPAR subunits GluA1 and GluA2, and Shank3 expression. (A) GluA1 mRNA abundance, represented as 2−ΔCt, increased across development (One-way ANOVA, p=0.0065, n=4), with a significant increase in expression at DIV19 compared to DIV5 (Tukey’s multiple comparisons, adjusted p=0.0050, n=4). (B) GluA2 mRNA abundance, represented as 2−ΔCt, increased across development (One-way ANOVA, p=0.0079, n=4), with a significant increase in expression at DIV19 compared to DIV5 (Tukey’s multiple comparisons, adjusted p=0.0066, n=4). (C) Shank3 mRNA abundance, represented as 2−ΔCt, was highly variable and did not change significantly across development (One-way ANOVA, p=0.4120, n=4). (D-E) Synaptosome-enriched Shank3 protein increased from the second week in culture (DIV14) to the third week in culture (DIV21) (unpaired t test, p=0.0285, n=3).