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. Author manuscript; available in PMC: 2024 Jul 13.
Published in final edited form as: Neurosci Lett. 2023 May 18;809:137305. doi: 10.1016/j.neulet.2023.137305

Figure 3:

Figure 3:

Effects of KCl treatment on synaptic GluA1, GluA2, or Shank3 protein levels and mRNA expression. (A-B) Overnight KCl treatment did not alter GluA1 synaptic protein levels (ratio paired t test, p= 0.7685, n=4) or (C) mRNA abundance, represented as 2−ΔCt (ratio paired t test, p=0.2982, n=4). The quantification represents summed intensity of the bands detected around 100 kD (expected molecular weight) and 80 kD. (D-E) Overnight KCl treatment also did not cause a change in Shank3 synaptic protein levels (ratio paired t test, p= 0.4250, n=4), or (F) mRNA expression (ratio paired t test, p=0.3379, n=4). (G-H) Overnight KCl treatment did not affect synaptic GluA2 protein levels (ratio paired t test, p=0.4509, n=4), but (I) did cause a 1.56-fold increase in GluA2 mRNA expression (ratio paired t test, p=0.0222, n=4).