Figure 4. Cilia length is normal in Arl13b^V358A/V358A kidneys, but ciliation rate is reduced in Arl13b^V358A/V358A cystic regions.

(A) Quantitation of cilia length in wild type kidneys as well as normal tubules and cystic regions of Arl13b^V358A/V358A kidneys, mean ± SEM. (B) Quantification of ciliation rate (cilia per basal body as indicated by acetylated alpha-tubulin and FGFR1OP (FOP) staining) in wild type kidneys and normal tubules and cystic regions of Arl13b^V358A/V358A kidneys, mean ± SEM. n = 3 mice per genotype with 100+ cilia counted per animal. One-way ANOVA, * p < 0.05. (C) Representative staining of acetylated tubulin and FOP in wild type kidneys and normal and cystic regions of Arl13b^V358A/V358A kidneys. Scale bars: 3 μm.