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. Author manuscript; available in PMC: 2024 Oct 1.
Published in final edited form as: Protein Expr Purif. 2023 Jun 6;210:106319. doi: 10.1016/j.pep.2023.106319

Figure 3. FPLC analysis of foam-fractionated lipid-free apoE4-NT and apoE4-NT rHDL.

Figure 3.

Gel filtration chromatography of a 300 μL aliquot of the eluate obtained following HiTrap Heparin HP chromatography of foamate (Panel A) and a 300 μL aliquot of foam-derived apoE4-NT-rHDL (Panel B). Samples were applied to a Superdex 200 Increase 10/300 column and eluted with 10 mM sodium phosphate, pH 3.4, at 0.75 mL/min with continuous monitoring of absorbance at 280 nm.