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. Author manuscript; available in PMC: 2024 Oct 1.
Published in final edited form as: Protein Expr Purif. 2023 Jun 6;210:106319. doi: 10.1016/j.pep.2023.106319

Figure 4. Characterization of apoE4-NT cholesterol efflux activity.

Figure 4.

Cholesterol efflux assays were performed using cholesterol-loaded and radiolabeled THP-1 monocyte / macrophages as described in Materials and Methods. Lipid-free apoA-I, lipid-free apoE4-NT (isolated from a shaker flask expression), and lipid-free, foamate-derived, apoE4-NT (F) were incubated with cells for 6 h at 37 °C. In a similar manner, efflux experiments were performed using apoA-I rHDL, shaker flask-derived apoE4-NT rHDL, and foamate derived apoE4-NT rHDL (F). Experiments were performed in triplicate, and control nonspecific efflux was subtracted from each condition to calculate apolipoprotein or rHDL specific efflux. Statistical significance was determined using one-way ANOVA multiple comparison with a Tukey’s post hoc test. Values reported are the mean ± standard deviation (n=3) *, p<0.05; **, p<0.001; ****, p<0.0001; ns, not significant.