Figure 1.

Sequence alignment of E.coli proteins from the RluA family (RluA, RluC and RluD) and S.cerevisiae YLR165c-encoded protein. The scale in amino acid residues is presented at the top. The three motifs (I, II and III) conserved in the RluA and RsuA families of RNA:Ψ-synthases are shown at the bottom. Identical and conserved residues are shaded. Aspartic acid residue mutated to alanine in the conserved motif II is indicated by an asterisk. Amino acid sequences of Motif II in different RNA:Ψ-synthase families are indicated at the bottom.