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. 2000 May 1;28(9):1941–1946. doi: 10.1093/nar/28.9.1941

Figure 2.

Figure 2

UsnRNA pseudouridylation pattern in the ΔYLR165c S.cerevisiae strain. Primer extension analysis was performed for U1 (A), U2 (B) and U5 (C) snRNAs from a total RNA fraction of the wild-type (WT) and ΔYLR165c S.cerevisiae strains. CMCT modifications were done for 1, 10 and 20 min (lanes 2, 3 and 4 respectively), experimental conditions are described in Materials and Methods. In lanes 3 and 4, the CMCT-modified RNA was subjected to an alkaline treatment at pH 10.4. A control extension experiment was made without addition of CMCT (lane 1). Lanes U, G, C and A correspond to the RNA sequencing ladder. Nucleotide positions, starting from the 5′-terminal nucleotide, are indicated on the right. The reverse transcription stops, corresponding to Ψ residues in U1, U2 and U5 snRNAs, are indicated by arrows on the left.