Table 1. Ψ formation in the extracts of wild-type and ΔYLR165c yeast strains.

Position tested in tRNA	tRNA transcript/labelling	Wild-type strain	ΔYLR165c strain
13	yeast tRNAAsp/ATP	0.75	0.65
32	yeast tRNAAsp/UTP	0.85	0.85
35	A.thaliana tRNATyr/ATP	0.55	0.65

Radiolabelled transcripts were incubated with yeast extract at 30°C for 60 min and the Ψ content was analysed by 2D-TLC after RNase T2 digestion.