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. Author manuscript; available in PMC: 2024 Jun 1.
Published in final edited form as: J Allergy Clin Immunol Pract. 2023 Apr 11;11(6):1579–1591. doi: 10.1016/j.jaip.2023.03.049

Figure 8: phosphor STAT staining.

Figure 8:

STATs become phosphorylated following activation by specific cytokines and this can be used to evaluate different cytokine/STAT pathways. For instance, (A) pSTAT1 is phosphorylated in monocytes from healthy controls following stimulation with IFNγ. However, (B) phosphorylation of STAT1 is defective in IFNGR1 or IFNGR2 deficient patients (Case 8). Similarly, (C) pSTAT3, (D) pSTAT4 and (E) pSTAT6 can be detected following stimulation with IL-21, IL-12 or IL-4, respectively (red histogram; control without cytokine: black histogram). Intracellular pSTATs are detected by flow cytometry using monoclonal antibodies to pSTAT specific residue following fixation and permeabilization of the cells.