Fig. 5.

All PBMCs were stimulated with LPS. Cytokine levels were compared with the Friedman test. The data are presented as median (IQR). Exposure to SN decreased the secretion of TNF-α (a) significantly (SN vs. control p = 0.0003, other comparisons ns) and increased the secretion of IL-10 (b) significantly (SN vs. control p < 0.0001, other comparisons ns). The data are here shown as ratios in relation to control. Sixteen individual PBMCs (represented by individual symbols in a, b) and four individual RBCs (each at storage day 5 and 33) were used in the assays. High individual variation of responder PBMCs cytokine secretion is seen. RBC storage age (c, d) did not affect cytokine secretion.