Figure 5.
Innate agonists synergize with a suboptimal dose of antibiotics to reduce B. abortus burdens in mouse spleens and lungs. a, b. Ampicillin was delivered to mice in the drinking water at the indicated doses starting at day 6 post infection with 105 CFU B. abortus. c, d. A dose of 100 mg/kg of ampicillin was delivered starting at day 6 post infection combined with lipo-CRX, 1μg i.t. on the day before infection (−1 dpi) and compared to vehicle, lipo-CRX or ampicillin alone. e, f. A combination treatment was also delivered i.t. at therapeutic intervals, 6 and 10 days after infection combined with a lower dose of ampicillin (50 mg/kg) and compared to vehicle, lipo-CRX or ampicillin alone. In each experiment, mice were euthanized 14 days post infection and bacterial burdens in spleens and lungs were assessed. Figures 5E and F are combined data from two different experiments, while A-D were single experiments with n = 5 mice per group. *p < 0.0 5, **p < 0.01 as calculated by the Mann-Whitney test.