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. 2023 Jun 26;14:1173544. doi: 10.3389/fimmu.2023.1173544

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Copyright © 2023 Steffan, Calise, Park, Niu, Yang, Hammock, Jones, Steele and Keller

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Differences in chemokines of G2A^-/- mice in comparison to wild-type. Cells were isolated from lungs of naïve, D1, and D2 samples post-infection of G2A^+/+ (blue bars) and G2A^-/- (orange bars) mice. Cells were incubated for 24h in modified IMDM. Supernatants were collected and analyzed using multiplex analysis to assess inflammatory chemokines MCP-1 (A), MIP-1α (B), MIP-1β (C), MIP-2(D), KC (E), and Eotaxin (F). Concentrations (pg/mL) are listed as average protein ± SEM from two independent studies pooled (individual values, circles). Statistical differences were assessed using the Student’s t test with Welch’s corrections with p values listed on graphs.