Figure 4.

No pathological differences in inflammation, but significant differences in the myeloid population including increased neutrophilia for G2A^-/- mice. Representative photomicrographs of lungs from G2A^+/+ Vh and A. fumigatus (Af) infected lungs (A, top) and G2A^-/- Vh and Af (A, bottom) are shown of H&E-stained samples. Cells isolated from lungs at D1 p.i. were assessed using flow cytometry in which populations of myeloid cells were categorized from total CD45+ cells. Percent of Ly6G⁺ cells (neutrophils, B), Ly6G^-CD11b⁺CD11c^- cells (monocytes, macrophages, and eosinophils, C), Ly6G^-CD11b^-CD11c⁺ (alveolar macrophages and dendritic cells, D), and Ly6G^-CD11b⁺CD11c⁺ (dendritic cells, E) from G2A^+/+ Vh or Af (blue bars) and G2A^-/- Vh or Af (orange bars) are shown as average percent ± SEM with individual samples (circles) shown. Statistical differences were determined using the Student’s t test with Welch’s correction. P values are listed within the graphs comparing groups.