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. Author manuscript; available in PMC: 2023 Jul 10.
Published in final edited form as: Cytokine. 2023 Feb 19;164:156159. doi: 10.1016/j.cyto.2023.156159

Fig. 3. Analysis of differentially expressed genes.

Fig. 3.

Messenger RNA of unstimulated, IL-4, IL-13 or IFN-γ stimulated neutrophils was analyzed using RNASeq. Differential gene expressions were tested applying DESeq2. (A) Signaling pathway impact analysis (SPIA). Two-dimensional plots illustrating the relationship between the two types of evidence considered by SPIA. The X-axis shows the over-representation evidence, while the Y-axis shows the perturbation evidence. Dots represent KEGG pathways. Red dots mark pathways significant at 5 % after Bonferroni correction, blue dots mark significantly enriched pathways (p < 0.05) according to FDR correction. (B) Heatmap of differentially expressed genes. Shown genes were selected based on differential expression (p < 0.05) and occurrence in only one KEGG pathway.