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. Author manuscript; available in PMC: 2023 Jul 10.
Published in final edited form as: Cytokine. 2023 Feb 19;164:156159. doi: 10.1016/j.cyto.2023.156159

Fig. 5. Effect of IL-4, IL-13 and IFN-γ on neutrophil energetics.

Fig. 5.

(A) Extracellular acidification (ECAR) was measured in milli-pH per minute (mpH/min) for 11 cycles. After measuring 3 basal level measurements, 3 measurements were recorded to determine the effect of Rotenone and Antimycin A (Rot/AA) for inhibiting mitochondrial respiration. Additional 5 measurements after injection of 2-deoxyglucose (2-DG) for glycolysis inhibition were detected. Used interval time between measurements was 8 min 30 sec. Representative curve for unstimulated and IL-4 stimulated neutrophils from one experiment is shown. (B) Comparison of relative basal glycolysis of neutrophils that were left unstimulated or stimulated with 50 ng/ml of IL-4, IL-13 or IFN-γ for one hour. The medians of each stimulation in each experiment (n = 6) are compared to unstimulated sample median of the same experiment. Averages and ± SEM are indicated. (C) Comparison of compensatory glycolysis from same samples as in 5B. The medians of each stimulation in each experiment (n = 6) are compared unstimulated sample median of the same experiment. Averages and ± SEM are indicated. *) p = 0.045; repeated measures ANOVA with Dunnett’s multiple comparison test used.