Fig.3.

Morphology and phenotypical characterization of hESC-derived lung progenitor cells at day 25 of differentiation. Phase-contrast images of cells and immunostaining for NKX2.1 and pro-surfactant protein C (pro-SFTPC) proteins in lung progenitors differentiated on A. Fibronectin (scale bar: 200 µm, 100 µm, 50 µm, respectively) and B. Hydrogel-coated plates (scale bar: 100 µm, 50 µm, 50 µm, respectively). C. H&E staining shows the attachment and retention of differentiated cells at day 25 (scale bar: 50 µm) and SEM photomicrograph shows the recellularization of scaffold surface reseeded with 15-day-old progenitor cells (scale bar: 20 µm). Immunohistochemistry for CK5, P63, pro-SFTPC, and AQP3 protein markers in the cells that were grown on the dECM scaffold at day 25 (scale bar: 50 µm, 50 µm, 50 µm, and 100 µm respectively). HESCs; Human embryonic stem cells and dECM; Decellularized extracellular matrix.