Table 1.
Strains and plasmids used in this work
| Strain or plasmid | Genotype/Description | Source or Ref. | |
|---|---|---|---|
| Strains | |||
| JPV # | |||
| 848 | WT BY4742 | MATα his3Δ1 leu2Δ0 lys2Δ0 ura3Δ0 | (86) |
| 846 | WT BY4742 GPC1-3xHA | BY4742, GPC1-3xHA | (23) |
| 865 | gpc1Δ | BY4742, gpc1::KanMX | (86) |
| 671 | hac1Δ | BY4742, hac1::KanMX | (86) |
| 878 | ire1Δ | BY4742, ire1::KanMX | (86) |
| 877 | ire1Δgpc1Δ | BY4742, ire1::KanMX gpc1::LEU2 | This study |
| 887 | hac1Δgpc1 | BY4742, hac1::KanMXgpc1::LEU2 | This study |
| 601 | ale1Δ | BY4742, ale1::KanMX | (86) |
| 888 | gde1Δ | BY4742, gde1::KanMX | (86) |
| 832 | gpc1Δale1Δ | BY4741, ale1::LEU2 gpc1::KanMX | (22) |
| 882 | WT pAM40-HDEL-DsRed | MATα containing integrative pAM40-HDEL-DsRed plasmid (JVE426) | This study; (40) |
| Plasmids | |||
| JVE # | |||
| 145 | pRS316 | Single-copy plasmid with URA3 | |
| 415 | pRS316-GPC1 | GPC1 and native promoter (750 bp upstream) inserted into pRS316 | This study |
| 421 | pRS313 | Single-copy plasmid with HIS3 | |
| 422 | pGT330 | IRE1 inserted into pRS313 | (5) |
| 423 | pGT201 | IRE1 with luminal domain deletion (Ire1ΔLD) inserted into pRS313 | (5) |
| 418 | p416-GIT3 | GIT3 inserted into p416, under the control of TEF1 promoter | This study |
| 426 | pAM40-HDEL-DsRed | Plasmid with URA3 marker and pRS306 backbone containing HDEL sequence as ER localization control | (40) |
| 393 | p416 | Multi-copy plasmid with URA3 marker and TEF1 promoter | |
| 392 | TEF1-GPC1-GFP | Plasmid with LEU2 marker, containing GPC1 under TEF1 promoter | This study |