Figure 5. Downregulation of Pebp1 prevents rAAV‐induced liver injury and hepatic necroptosis in db/db mice.

• A
  The top 15 hepatic genes upregulated in 9‐week‐old db/db mice compared with wild‐type mice. n = 1, liver samples from three mice were mixed together in equal amounts for RNA extraction.
• B
  Relative mRNA levels of Cyp4a14, Pebp1 and Tat in livers of wild type and db/db mice. n = 6 for each group.
• C
  Schematic of the experimental design to study the role of candidate genes in liver injury and hepatic necroptosis induced by rAAV.
• D–F
  Body weight (D), liver weight (E), and blood glucose levels (F) of db/db mice after a single injection of rAAV‐si‐NC/Cyp4a14/Pebp1/Tat for 2 months. n = 10–12. rAAV‐si‐NC was packaged as indicated in Fig 1A and mentioned as rAAV in Figs 1, EV1, EV2, EV3, 2, EV4, 3, 4.
• G, H
  Serum ALT (G) and AST (H) activities of mice in (D).
• I
  Liver images, H&E staining of liver sections and the incidence of death and hepatic necroptosis (Ncp) of mice in (D). n = 10–14, NML, Normal.
• J
  The indicated hepatic mRNA levels of mice in (D).
• K
  The hepatic p‐MLKL level of the indicated mice in (D).
• L
  The indicated hepatic mRNA levels of mice in (K).

Data information: In (B, D–H, J–L), Data are presented as mean ± SD. Student's t‐test.

Source data are available online for this figure.