Figure 2.
LncRNA SNHG20 knockdown inhibited HCC cell proliferation while accelerating apoptosis. Huh-7 and HepG2 cells were transfected with sh-LncRNA SNHG20, with sh-NC acting as the negative control, in order to knock down LncRNA SNHG20. A: To measure the knockdown efficiency of LncRNA SNHG20, RT-qPCR was adopted. B: Cell viability of Huh-7 and HepG2 cells was determined using CCK-8 assay. C, D: EdU assay (magnification, ×200) and western blot were conducted to measure the proliferation ability of Huh-7 and HepG2 cells. E, F: FCMs assay and western blot were conducted to evaluate the apoptosis of Huh-7 and HepG2 cells. Data are the average of three independent experiments. The error bars show SD. In contrast with the sh-NC group, **P<0.01. sh-NC was used as the negative control. LncRNA: long non-coding RNA.
