Figure 3.

LncRNA SNHG20 knockdown prevented HCC cells from migrating and invading. Huh-7 and HepG2 cells were transfected with sh-LncRNA SNHG20, with sh-NC acting as the negative control, to knock down lncRNA SNHG20. A: Huh-7 and HepG2 cell movement was evaluated using a wound-healing migration assay (magnification, ×200); B: Transwell assay was implemented to quantify the invasion and migration of Huh-7 and HepG2 cells (magnification, ×200); C: Western blotting was applied to ascertain the concentrations of proteins related to the invasion ability, such as Cox-2, MMP-2, and MMP-9. Data are the average of three independent experiments. The error bars show SD. In contrast with the sh-NC group, **P<0.01. sh-NC was used as the negative control. lncRNA: long non-coding RNA.