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. 2023 Jun 15;15(6):4314–4331.

Figure 6.

Figure 6

miR-5095 negatively targeted MBD1. A: miRDB program was able to determine the probable binding location between miR-5095 and MBD1. B: Huh-7 and HepG2 cells were transfected with miR-5095 mimics, with NC mimics acting as a negative control. To validate the direct combining interaction between miR-5095 and MBD1, a dual luciferase reporter gene test was carried out; C: Huh-7, MHCC97-H, HepG2, and SMMC-7721 cells were used as HCC cell lines in RT-qPCR to identify the MBD1 expression, and HL-7702 cells were used as the negative control; D: MBD1 expressions in Huh-7 and HepG2 cells were measured using RT-qPCR in response to miR-5095 overexpression. E: Huh-7 and HepG2 cells that had miR-5095 overexpressed were used to determine the level of MBD1 protein. The data represent the average of three independent experiments. The error bars indicate SD. In contrast to the NC mimics group, **P<0.05, ***P<0.001. NC mimics was used as the negative control. MBD1: Methyl-CpG-Binding Domain Protein 1.