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. 2023 Jun 15;15(6):4314–4331.

Figure 7.

Figure 7

LncRNA SNHG20 regulated HCC progression through the miR-5095/MBD1 axis. In Huh-7 and HepG2 cells, miR-5095 inhibitor or sh-MBD1 was transfected to suppress the LncRNA SNHG20 for 24 hours; the negative control for the miR-5095 inhibitor was an NC inhibitor, while the negative control for sh-MBD1 was sh-NC. A: MBD1 or miR-5095 levels were determined using RT-qPCR; B: Huh-7 and HepG2 cell viability was assessed with CCK-8. C: EdU test was conducted (magnification, ×200) to evaluate the proliferation of Huh-7 and HepG2 cells. D: Huh-7 and HepG2 cell apoptosis was assessed using the FCMs assay. E: Transwell experiment was conducted to evaluate the invasion and migration of Huh-7 and HepG2 cells (magnification, ×200). Data are the average of three independent experiments. The error bars reflect SE. In contrast to the NC inhibitors group, *P<0.05, **P<0.001; in contrast to the miR-505 inhibition group, #P<0.05. sh-NC, NC inhibitors or NC inhibitors + sh-NC was used as the negative control. WT: wild type; MUT: mutated. LncRNA: long non-coding RNA. MBD1: Methyl-CpG-Binding Domain Protein 1.