Figure 4.

Regulation of the expression of p-Akt and the NF-κB p50 subunit of (–)-cryptanoside A (1). MDA-MB-231 cells were incubated with 1 or digoxin for 5 h, at concentrations of 0.5 μM and 0.48 μM, respectively. Expression of p-Akt and the NF-κB p50 subunit was determined by western blotting using rabbit monoclonal cleaved antibodies, and data for the targeted protein were normalized by the corresponding β-actin results of the same samples. A representative blot is shown from three independent experiments with similar results being shown (Experimental Section) [columns, mean in each group (n ≥ 3); bars, SE; *** p ≤ 0.05 and **** p ≤ 0.01 when compared to the control group (ctrl.)].