Figure 5.

Regulation of the expression of Akt, the NF-κB p50, p52, and p65 subunits, and PI3K of 1. MDA-MB-231 cells were incubated with (–)-cryptanoside A (1) or digoxin for 72 h at different concentrations. Expression of the proteins tested was determined by western blotting using rabbit monoclonal cleaved antibodies, and data for the targeted proteins were normalized by the corresponding β-actin results of the same samples. A representative blot is shown from three independent experiments with similar results being shown (Experimental Section) [columns, mean in each group (n ≥ 3); bars, SE; * p ≤ 0.5, ** p ≤ 0.1, *** p ≤ 0.05, and **** p ≤ 0.01 when compared to the control group (ctrl.) or digoxin].