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Purification table of CPV-2 endonuclease expressed in E. coli BL21 (De3) pLysS.
| Steps | Total volume (ml) | Protein (mg/ml) | Total protein (mg) | Total activity (FC)* | Specific activity (FC/mg) | Purification fold | Yield (%) |
|---|---|---|---|---|---|---|---|
| Culture supernatant | 100 | 6.75 | 675 | 120 × 104 | 1777.78 | 1 | 100 |
| Ni-NTA purification | 3 | 0.40 | 1.2 | 64.6 × 104 | 538333 | 302.81 | 53.83 |
*FC (Fluorescence concentration) (FC=RFU·min-1 × D × V) (D: dilution factor, V: volume)
