Table 2.

Inhibitory activities of the natural compounds against CPV-2 endonuclease at 50 μM.

Group	Compound	Inhibition (%) at 50 μM
Diarylheptanoid	Curcumin	100
	Bisdemethoxycurcumin	100
	Demethoxycurcumin	100
Hydroxycinnamic acid	Ferulic acid	37 ± 5
	Chlorogenic acid	29 ± 4
	Caffeic acid	42 ± 8
Triterpenoid	Oleanolic acid	51 ± 7
	Ursolic acid	29 ± 5
Flavonol	Myricetin	0
	Fisetin	0
	Rutin	0
	Quercetin	51 ± 3
	Astragalin	0
Flavanone	Naringenin	0
Isoflavone	Genistein	45 ± 14
	Daidzein	22 ± 12
Flavan-3-ol	EGCG	49 ± 6
Furanoid lignan	(+)-Sesamin	0
Benzodioxole	Sesamolin	0
	Sesamol	9 ± 5
Alkaloid	Piperine	0
	Caffeine	7 ± 7
Tannoid	Tannic acid	100
Fatty acid	Linoleic acid	100
Tocopherol	α-Tocopherol (Vitamin E)	98 ± 3
	Vitamin K1	68 ± 15
Butenolide	L-Ascorbic acid	0
	Gallic acid	2 ± 2
Xanthonoid	Mangiferin	0
Stilbenoid	Resveratrol	0
	L-carnitine	29 ± 2
Mixture	Extracted sesame cake at 100 μg/ml	100
	Extracted tumeric at 10 μg/ml	95 ± 2
	Extracted yerba mate at 40 μg/ml	95

The reaction mixture contained 0.6 μM FRET substrate, 1.25 μg of enzyme, and 50 μM inhibitor in 50 mM Tris/HCl (pH 7.0).

The reaction was run at 37°C for 3 min.

Mean ± standard error of the mean (SEM)