Fig. 3. Disruption of established microbial biofilm from Pseudomonas aeruginosa after treatment with a nutraceutical enzyme and botanical blend across a dose range of 0.5 – 30 mg/ml.

Data is shown as the average ± standard deviation of a minimum of 3 repeats of each treatment dose, as the % change from untreated biofilm, where the untreated biofilm had gone through identical procedures for removal of planktonic forms, washing, and addition of fresh medium. Biofilm mass was quantified by crystal violet staining (solid lines), and the metabolic activity of the microbial biofilms was measured by the MTT assay (dashed lines). Slime formation was scored with “3” (300%) change indicating that each culture in the microtiter plate had turned to a mucus plug. All data points were statistically significant when compared to the untreated control biofilm cultures, except where the data point is annotated by NS (not significant).