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. Author manuscript; available in PMC: 2023 Jul 10.
Published in final edited form as: Nat Mater. 2022 Dec 1;22(1):117–127. doi: 10.1038/s41563-022-01400-4

Extended Data Figure 9. Organoids grow, break symmetry and form buds with time.

Extended Data Figure 9.

a, Examples of growth of intestinal organoids in elastic versus viscoelastic hydrogels over 7 days. Phalloidin in cyan, Hoechst in magenta. Scale bar is 100 μm. b, Quantification of organoid circularity in different stiffness elastic and viscoelastic hydrogels. n=32,32,38,37 organoids per condition. Statistical analysis was performed using Kruskal–Wallis test followed by post hoc Dunn’s test. Data represent mean ± s.d.