Table 1.

Bacterial strains, plasmid, and primers used in this study

Name	Description	Reference or source
Agrobacterium vitis - Bacterial Strain
S4	Wild type	T. J. Burra
ΔhnoX	S4 avi_3098::pVIK165, KmR	T. J. Burra
ΔhnoX/phnoX	ΔhnoX mutant containing pPZP201-avi_3098, KmR, SpR	T. J. Burra
Escherichia coli - Bacterial Strain
BL21(DE3) pLysS	Expression strain, CmR
DH5$\alpha$	Plasmid amplification
S17–1/$\lambda$pir	Host Strain for biparental mating	(40)
Plasmid
pET20b	Cloning vector, AmpR	Novagen
pET20b-avi_3097 WT	pET20b carrying avi_3097 WT, AmpR	This work
pET20b -avi_3098 WT	pET20b carrying avi_3098 WT, AmpR	This work
pET20b-avi_3097 GGAAF	pET20b carrying avi_3097 GGAAF, AmpR	This work
pET20b-avi_3097 AAL	pET20b carrying avi_3097 AAL, AmpR	(39)
pVIK165	Suicide vector, KmR	(41)
pPZP201	Broad-host range cloning vector, SpR	(42)
pPZP201-avi_3098 (phnoX)	pPZP201 carrying avi_3098, SPR	This work
Complementation - Primers
avi_3098 FWD comp	TAGAGGATCCATGAAAGGGATGGTTTTTACGGAAATG	This work
avi_3098 REV comp	AGATCTGCAGTCACGATGCGCTCTCGGCGATC	This work
Amplification of avH-NOX and avHaCE WT from Agrobacterium vitis S4 genomic DNA - Primers
avi_3097 WT FWD	GGGCCCGCGCATATGGTAAACCAGCCATTTGAG	(39)
avi_3097 WT REV	CCCGGGCGCCTCGAGAGGGCTCTTTCCAGCTGCAAGGCT	(39)
avi_3098 WT FWD	GGGCCCGCGCATATGAAAGGGATGGTTTTTACG	This work
avi_3098 WT REV	CCCGGGCGCCTCGAGCGATGCGCTCTCGGCGATCAC	This work
Site-directed mutagenesis - Primers
avi_3097 GGAAF FWD	GTGGCACGGCTGGGTGGTGCCGCCTTTGCATTGATTCTCGAC	This work
avi_3097 GGAAF REV	GTCGAGAATCAATGCAAAGGCGGCACCACCCAGCCGTGCCAC	This work
avi_3097 AAL FWD	CGGATACTGGGGGTGGCCGCCCTGGCACGCTGGCAGCAT	(39)
avi_3097 AAL REV	ATGCTGCCAGCGTGCCAGGGCGGCCACCCCAGTATCCG	(39)

^aGift from Thomas Burr at Cornell University, Geneva, NY, USA