. Author manuscript; available in PMC: 2023 Jul 10.

Published in final edited form as: Biochemistry. 2023 Feb 6;62(4):912–922. doi: 10.1021/acs.biochem.2c00639

TABLE 2.

Ligand binding properties of H-NOX proteins from multiple bacterial species: electronic absorption maxima and NO dissociation rate constants

H-NOX Proteins	$λ$ _max (nm) ( $α / β$ ) (nm)			k_off (NO) (x10⁻⁴ s⁻¹)
H-NOX Proteins	Fe(II)	Fe(II)-CO	Fe(II)-NO	k_off (NO) (x10⁻⁴ s⁻¹)
av ^a	429 (567)	422 (542/571)	398 (544/567)	8.2 $\pm$ 3.0
sw ^b	430 (ND)	423 (ND)	399 (ND)	15.2 $\pm$ 3.5
Lpg1 ^c	428 (557)	420 (540/566)	398 (540/571)	10.3 $\pm$ 1.4
sGC^d	431 (555)	423 (541/567)	398 (537/572)	8.2 $\pm$ 2.0^e

This study;

Ref. (12);

Ref. (44);

Ref. (48);

Ref. (49);

av = A. vitis H-NOX (avi_3098); sw = S. woodyi H-NOX (swoo_2751); lpg1 = L. pneumophila H-NOX (lpg1056) Legionella pneumophila; ND = not determined