TABLE 3.

Steady-state kinetic parameters for AAL (DGC activity) and GGAAF (PDE activity) variants of AvHaCE

Enzyme	kcat (s−1)	KM (μM)	$\frac{{\mathit{k}}_{\mathbf{c}\mathbf{a}\mathbf{t}}\mathbf{ }}{{\mathbf{K}}_{\mathit{M}}}\mathbf{ }(\mathbf{s}-1\mathbf{ }\mathbf{\mu }\mathbf{M}-1)\mathbf{ }\mathbf{x}\mathbf{ }10-1$
AAL	0.031 ± 0.001	17.0 ± 0.72	0.018 ± 0.001
GGAAF	0.036 ± 0.002	5.2 ± 0.9	0.068 ± 0.001