KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Tristetraprolin | Millipore | ABE285 |
| Tristetraprolin | Cell Signaling Technology | Cat# 71632; RRID:AB_2799806 |
| BRF1/2 | Cell Signaling Technology | Cat# 2119; RRID:AB_10695874 |
| ERK | Cell Signaling Technology | Cat# 9102; RRID:AB_330744 |
| phospho-Thr202/Tyr204 ERK | Cell Signaling Technology | Cat# 4370; RRID:AB_2315112 |
| S6 kinase | Cell Signaling Technology | Cat# 2708; RRID:AB_390722 |
| phospho-T389 S6 kinase | Cell Signaling Technology | Cat# 9234; RRID:AB_2269803 |
| β-actin | Cell Signaling Technology | Cat# 3700; RRID:AB_2242334 |
| c-Myc | Cell Signaling Technology | Cat# 18583; RRID:AB_2895543 |
| GAPDH | Cell Signaling Technology | Cat# 5174; RRID:AB_10622025 |
| α-tubulin | Sigma | Cat# T6074; RRID:AB_477582 |
| Recombinant Anti-Heme Oxygenase 1 antibody | Abcam | Cat# ab68477 |
| CD31 | BD Biosciences | Cat# 557355; RRID:AB_396660 |
| Donkey Anti-Mouse IgG H&L (Alexa Fluor® 568) preadsorbed | Abcam | Cat# ab175700 |
| Donkey anti-Rat IgG (H + L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 488 | Thermo Fisher Scientific | Cat# A-21208; RRID:AB_2535794 |
| ENO2 | ABFrontier | Cat# LF-MA0097 |
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| Bacterial and virus strains | ||
|
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| Human Adenovirus Type5 (dE1/E3) | Vector Biolabs | Cat. # 1045/1060 |
|
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| Chemicals, peptides, and recombinant proteins | ||
|
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| Acid-Phenol:Chloroform, pH 4.5 (with IAA, 125:24:1) | Thermo Fisher Scientific | Cat# AM9720 |
| Proteinase K | NEB | Cat# P8107S |
| Lipofectamine 3000 | Thermo Fisher Scientific | Cat# L3000001 |
| RNase inhibitor | NEB | M0314S |
| RNase I | Thermo Fisher Scientific | EN0601 |
| Turbo DNase | Thermo Fisher Scientific | AM2238 |
| U-13C6-glucose | Cambridge Isotope Laboratories | Cat# CLM-1396–1 |
| Polybrene | Santa Cruz | Cat# sc-134220 |
| PolyFect Transfection Reagent | Qiagen | Cat# 220002–078 |
| Blasticidin | Invivogen | Cat# ant-bl-1 |
| PD 0325901 | Sigma | Cat# PZ0162 |
| SB 203580 | Sigma | Cat# S8307 |
| Torin-1 | Tocris | Cat# 4247 |
| Cyclohexamide | Sigma | Cat# 01810 |
| Actinomycin D | Sigma | Cat# A1410 |
| Recombinant Human EGF Protein | Thermo Fisher Scientific | Cat# PHG0313 |
| Recombinant Human FGF-basic | Peprotech | Cat# 100–18B |
| Human recombinant insulin | Thermo Fisher Scientific | Cat# 12585014 |
| Recombinant Human IGF-I | Peprotech | Cat# 100–11 |
| Recombinant Human PDGF-BB | Peprotech | Cat# 100–14B |
| ProLong Gold Antifade Mountant | Thermo Fisher Scientific | Cat# P36930 |
| Dynabeads™ Protein A | Thermo Fisher Scientific | Cat# 10001D |
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| Critical commercial assays | ||
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| Promega DualGlo Luciferase Assay System | Promega | Cat# E2920 |
| iScript™ Reverse Transcription Supermix | Bio Rad | Cat# 1708841 |
| Pierce™ BCA Protein Assay Kit | Thermo Fisher Scientific | Cat# 23227 |
| ΔRNA Clean & Concentrator-5 | Zymo Research | Cat# R1013 |
| Q5 PCR Master Mix | NEB | Cat# M0492S |
| AffinityScript reverse transcriptase | Agilent Technologies | Cat# 600107 |
| T4 PNK | NEB | Cat# M0201L |
| Applied Biosystems™ Power SYBR™ Green PCR Master Mix | Applied Biosystems | Cat# 4368706 |
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| Deposited data | ||
|
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| RNA-seq | NCBI Sequence Read Archive | SRA: PRJNA942602 |
| eCLIP-seq | NCBI Sequence Read Archive | SRA: PRJNA943291 |
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| Experimental models: Cell lines | ||
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| A549 (human lung adenocarcinoma) | Steven Dubinett (University of California, Los Angeles) | RRID: CVCL_0023 |
| HeLa (human cervical adenocarcinoma) | Steven Bensinger (University of California, Los Angeles) | RRID: CVCL_0030 |
| MCF-10A (human breast epithelial) | Frank McCormick (University of California, San Francisco) | RRID: CVCL_0598 |
| 293T (human embryonic kidney) | Steven Bensinger (University of California, Los Angeles) | RRID: CVCL_0063 |
| HUVECs | M. Luisa Iruela-Arisp (University of California, Los Angeles) | RRID:CVCL_2959 |
| HFF-1 | ATCC | RRID:CVCL_3285 |
| Zfp36/l1/l2 triple-floxed MEFs (mouse embryonic fibroblasts) | Martin Turner (The Babraham Institute, Cambridge) | Hodson et al., 2010; New et al., 2017. |
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| Experimental models: Organisms/strains | ||
|
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| Mouse strain: C57BL/6J | The Jackson Laboratory | Cat# 000664 |
| Mouse strain: Tg(Cdh5-cre/ERT2)-Zfp36flox/flox Gt(ROSA)26Sor tm14(CAG tdTomato)Hze) | M. Luisa Iruela-Arisp (Northwestern University) | Sorensen et al., 2009; Qiu, L. Q. et al., 2012; Lizama et al., 2015. |
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| Oligonucleotides | ||
|
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| sgRNA targeting ROSA26: GTAGTAATGAGAGTTCACTG | This paper | N/A |
| sgRNA targeting mouse Eno2 #1: ATAGAGATCCACCTCCACGG | This paper | N/A |
| sgRNA targeting mouse Eno2 #2: GATTTGGGCCCGAGAGATCT | This paper | N/A |
| qPCR primers (Table 1) | NCBI Primer-BLAST | https://www.ncbi.nlm.nih.gov/tools/primer-blast/ |
| Site directed mutagenesis primers (Table 2) | Agilent QuickChange II primer design | https://www.agilent.com/store/primerDesignProgram.jsp |
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| Recombinant DNA | ||
|
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| PCMV6-Kan/Neo-Mouse Eno2 | Origene | Cat# MC201508 |
| pMirTarget vector | Origene | Cat# PS100062 |
| CMV-ZFP36 | This paper | N/A |
| Renilla Luciferase | William Lowry (University of California, Los Angeles) | Cinkornpumin et al., 2017. |
| lentiCRISPR v2-Blast | Addgene | Cat# 83480 |
| eGFP Adenovirus | Vector Biolabs | Cat# 1060 |
| Cre Recombinase Adenovirus | Vector Biolabs | Cat# 1045 |
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| Software and algorithms | ||
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| Prism8 | GraphPad | https://www.graphpad.com/scientificsoftware/prism/ |
| Image J | NIH | https://imagej.nih.gov/ij/ |
| Imaris 9.9.0 | GitHub | https://github.com/topics/imaris |
| MZmine 2 | GitHub | http://mzmine.github.io/ |
| AccuCor | GitHub | https://github.com/lparsons/accucor |
| STAR | GitHub | https://github.com/alexdobin/STAR |
| DESeq2 | GitHub | https://github.com/mikelove/DESeq2 |
| UMI-tools | GitHub | https://github.com/CGATOxford/UMI-tools |
| PureCLIP | GitHub | https://github.com/skrakau/PureCLIP |
| clusterProfiler | GitHub | https://github.com/YuLab-SMU/clusterProfiler |
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| Other | ||
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| QuantStudio5 | Thermo Fisher Scientific | N/A |
| Z1 Particle Counter | Beckman Coulter | N/A |
| A1R HD25 confocal microscope | Nikon | N/A |
| NOVASeq 6000 | Illumina | N/A |
| Bioruptor | Diagenode | UCD-200 |
| Q Exactive | Thermo Fisher Scientific | N/A |