| Antibody |
anti-HIF-1α (Mouse monoclonal) |
BD Biosciences |
610958 |
1:1000 |
| Antibody |
anti-c-MYC (Rabbit monoclonal) |
Cell Signaling Technologies |
D84C12 |
1:1000 |
| Antibody |
anti-LDHA (Rabbit polyclonal) |
Cell Signaling Technologies |
2012 |
1:1000 |
| Antibody |
HRP-anti-Rabbit IgG (Goat polyclonal) |
Cell Signaling Technologies |
7074 |
1:5000 |
| Antibody |
HRP-anti-Mouse IgG (Goat polyclonal) |
Cell Signaling Technologies |
7076 |
1:5000 |
| Transfected construct (human) |
MYC |
Vector Biolabs |
1285 |
adenovirus to express MYC |
| Transfected construct (human) |
YFP |
Oldham et al., 2015
|
|
adenovirus control to express YFP |
| Chemical compound, drug |
[1,2–13 C2]-glucose |
Cambridge Isotope Labs |
CLM-504-PK |
|
| Chemical compound, drug |
[U-13C6]-glucose |
Cambridge Isotope Labs |
CLM-1396-PK |
|
| Chemical compound, drug |
[U-13C5]-glutamine |
Cambridge Isotope Labs |
CLM-1822-H-PK |
|
| Chemical compound, drug |
[U-13C3]-lactate |
Sigma |
485926 |
|
| Chemical compound, drug |
BAY |
Cayman |
15297 |
Molidustat (BAY-85–3934); 10 μM in DMSO |
| Commercial assay or kit |
Glucose colorimetric assay kit |
Cayman |
10009582 |
|
| Commercial assay or kit |
ʟ-Lactate assay kit |
Cayman |
700510 |
|
| Commercial assay or kit |
Pyruvate assay kit |
Cayman |
700470 |
|
| Commercial assay or kit |
NADP/NADPH-Glo Assay |
Promega |
G9081 |
|
| Cell line (human) |
LFs |
Lonza |
CC-2512 |
Normal human lung fibroblasts |
| Cell line (human) |
PASMCs |
Lonza |
CC-2581 |
Pulmonary artery smooth muscle cells |
| Sequence-based reagent |
ACTB |
Life Technologies |
Hs03023943_g1 |
qPCR probe |
| Sequence-based reagent |
GLUT1 |
Life Technologies |
Hs00892681_m1 |
qPCR probe |
| Sequence-based reagent |
LDHA |
Life Technologies |
Hs00855332_g1 |
qPCR probe |
| Sequence-based reagent |
CDKN1A |
Life Technologies |
Hs00355782_m1 |
qPCR probe |
| Transfected construct (human) |
siMYC |
Dharmacon |
L-003282-02-0005 |
ON-TARGETplus siRNA |
| Transfected construct (human) |
siPHD2 |
Dharmacon |
L-004276-00-0005 |
ON-TARGETplus siRNA |
| Transfected construct (human) |
siCTL |
Dharmacon |
D-001810-10-05 |
ON-TARGETplus non-targeting control pool |
